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Table 2 Polymerase chain reaction primers

From: Identification of subpopulations with characteristics of mesenchymal progenitor cells from human osteoarthritic cartilage using triple staining for cell surface markers

Target

Primers

AP

5'-ACC TCG TTG ACA CCT GGA AG-3'

 

5'-CCA CCA TCT CGG AGA GTG AC-3'

BSP

5'-TGC ATT GGC TCC AGT GAC ACT-3'

 

5'-TGC TCA GCA TTT TGG GAA T-3'

Col1

5'-TAA CTT CTG GAC TAT TTG CGG ACT TTT GG-3'

 

5'-CAA CCT CAG CCC ATT GGC GCT G-3'

GAPDH

5'-CGG AGT CAA CGG ATT TGG TCG TAT-3'

 

5'-AGC CTT CTC CAT GGT TGG TGA AGA C-3'

OCN

5'-CTG GCC CTG ACT GCA TTC TGC-3'

 

5'-AAC GGT GGT GCC ATA GAT GCG-3'

  1. For primer design, Primer3 was used (Rozen S, Skaletsky HJ [1998; available at http://www-genome.wi.mit.edu/genome_software/other/primer3.html]), with published DNA sequences from GenBank (NCBI). Used parameters: product size 180–600 base pairs, annealing temperature 60°C, and primer length 18–30 base-pairs. AP, alkaline phosphatase; BSP, bone sialoprotein; Col1, collagen type I; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; OCN, osteocalcin.