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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Children with oligoarticular juvenile idiopathic arthritis have skewed synovial monocyte polarization pattern with functional impairment—a distinct inflammatory pattern for oligoarticular juvenile arthritis

Fig. 1

Synovial fluid monocytes express both M1- and M2-related polarization markers at the surface level. Synovial fluid and paired blood samples were collected from patients with oligoarticular JIA and analyzed by flow cytometry. a Monocyte subset distribution in the blood and synovial fluid based on CD14 and CD16 expression, n = 13, ***p < 0.001. b Representative gating plots based on CD14 and CD16 as summarized in a, for circulating- and synovial monocytes, respectively. Given that CD14-positive monocytes represent the majority of monocytes in synovial fluid, they were gated for further analysis. Compared to circulating monocytes, synovial monocytes expressed high levels of the c M1-like marker CD40 median (IQR): 2452 (895–3213) vs 688 (253–1066), CD86 6414 (4628–8328) vs 3247 (2561–3872), and the M2 marker CD206 310 (232–432) vs 88 (75–98). There was no difference in the expression of CD163 235 (168–303) vs 268 (217–406). Statistics was performed using Wilcoxon matched-pairs signed rank test (n = 13, ***p < 0.001). Data are shown as median. MFI-median fluorescence intensity

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Arthritis Research & Therapy

ISSN: 1478-6362

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