Fig. 4
Localisation and expression of cytokine-regulated, stress granule protein hnRNP-DLmir in cells, and synovial tissue. Anti-human hnRNP-DLmir antibodies detect stress granules in immunofluorescence microscopy. Staining with an affinity-purified α-human hnRNP-DLmir antibody was performed in HEp-2 (a) and HeLa cells (b). HeLa cells were treated with 0.5 mM sodium arsenite to induce stress granules and stained with affinity-purified α-human hnRNP-DLmir antibodies (c), mouse α-human ATXN2 antibodies 63 (d), mouse α-human hnRNP-A2/B1 antibodies (e) and α-human AUF1 peptide-specific rabbit serum 19 (f). Co-localization of AUF1 and stress granules/P-bodies. Staining of HEp-2 cells with α-RCK/p54 64 antibodies (g) and double staining of HEp-2 cells with affinity-purified α-human AUF1 (green) and α-RCK/p54 64 antibodies (red) (h). Merged sections are visible in yellow. B Expression of hnRNP-DL in synovial tissue from a patient with rheumatoid arthritis, a patient with osteoarthritis and a healthy subject, each in 20-fold and detail in 40-fold magnification