Fig. 3From: Expansion of myeloid-derived suppressor cells contributes to metabolic osteoarthritis through subchondral bone remodelingHigh-fat diet (HFD) increases osteoclastogenesis in DMM mice. A Representative images of isolated bone marrow M-MDSC post-M-CSF and RANKL stimulation stained with tartrate-resistant acid phosphatase (TRAP; n = 3 in each group). B Osteoclast number (n = 3 in each group), number of osteoclasts per field of view, each enumeration utilized 5 fields of view/slide at 20×. C Osteoclast nuclei number per osteoclast (n = 3 in each group). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by two-way ANOVA Tukey’s multiple comparison testBack to article page