Fig. 3

Manual gating overlay of FlowSOM tree for CCR6⁺ and CCR6^- Th cell subpopulations. PBMCs of psoriasis and PsA patients and healthy controls were isolated and used for Flow cytometry. Doublets and dead cells were excluded. Cells were analyzed as described in Fig. 1. A Cells that were gated manually as CCR6⁺ or CCR6^- are indicated with yellow or green on the FlowSOM tree. B Cells that were gated manually as CCR6 subpopulations (Th17, Th22, Th17.1, DP, DN, or Th1 and Th2) are indicated by colors on the FlowSOM tree