Fig. 2

Osteogenic potentials of SF subsets. A Representative photographs of whole dishes stained with alizarin red and ALP. Individual subsets were incubated in the calcification differentiation medium for 3 weeks. B Quantification of the staining. Cells per donor were cultured in 3 dishes and the results of 10 donors for alizarin red and 11 donors for ALP were plotted. C Gene expression levels by qPCR. The expression levels of the genes were plotted as a ratio to the expression level of 18S of undifferentiated SF as a control. Cells per donor were cultured in 3 dishes and the results of 10 donors for RUNX2 and ALPL and 9 donors for OCN. OA and RA samples are plotted as white circles and white squares, respectively. The average data of each subset are plotted as black squares. Data were analyzed by Dunn’s multiple comparison test for comparing each subset from the same patients (*p < 0.05, **p < 0.01)