Table 1 Gene primer sequences of rats used for qRT-PCR
Genes | Forward primer | Reverse primer |
|---|---|---|
Col2a1 | GGAGCAGCAAGAGCAAGGAGAAG | TCAGTGGACAGTAGACGGAGGAAAG |
Aggrecan | AGAAGAGACCCAAACAGCAGAAACAG | GCAGGTGGCTCCATTCAGACAAG |
MMP13 | CAAGATGTGGAGTGCCTGATGTGG | GCGTGTGCCAGAAGACCAGAAG |
Runx2 | CTTCGTCAGCGTCCTATCAGTTCC | TCCATCAGCGTCAACACCATCATTC |
Osteocalcin | GGACCCTCTCTCTGCTCACTCTG | ACCTTACTGCCCTCCTGCTTGG |
CD63 | CGGTGGAAGGAGGAATGAAGTGTG | AACCTGAACTGCTACGCCAATGG |
Mgp | CAGCAGAGGTGGCGAGCTAA | ATGGCGTAGCGCTCACACAG |
Npp1 | AGGGCATTCACACGGACCA | CTTCAGGCCATCCATCAGCA |
Tnap | CACGTTGACTGTGGTTACTGCTGA | CCTTGTAACCAGGCCCGTTG |
Gapdh | GGCACAGTCAAGGCTGAGAATG | ATGGTGGTGAAGACGCCAGTA |