Fig. 1
From: Dark-light cycle disrupts bone metabolism and suppresses joint deterioration in osteoarthritic rats
Body growth of normal and OA rats under LD and DL cycle. A LD and DL cycle protocol. B Body weight gain of rats at the end of study. Two-way ANOVA: LD factor: F(1, 24) = 51.81, P < 0.0001; model factor: F(1, 24) = 9.408, P = 0.0053; n = 7 per group. C Linear regression of body weight gain and weeks in each group. D Safranin O and fast green staining for tibia growth plates of normal and OA rats. RZ, resting zone; PZ, proliferating zone; HZ, hypertrophy zone. Scale bar, 50 μm. Two-way ANOVA: Total length: LD factor: F(1, 8) = 25.52, P = 0.0010; RZ: LD factor: F(1, 8) = 13.94, P = 0.0058; PZ: LD × model: F(1, 8) = 8.507, P = 0.0194; HZ: LD factor: F(1, 8) = 38.25, P = 0.0003; n = 3 per group