Fig. 4

Significant activity of the bone marrow MPS after LD reversal. A Presentative images of proliferation of tibial marrow cells stained by Ki67 (white arrow) and DAPI. Scale bar, 50 μm and 20 μm. Two-way ANOVA: LD factor: F_{(1, 8)} = 108.6, P < 0.0001; model factor: F_{(1, 8)} = 5.332, P = 0.0498; n = 3 per group. B Presentative images of F4/80⁺ macrophages (white arrow) of tibia. Scale bar, 50 μm. Two-way ANOVA: LD factor: F_{(1, 8)} = 21.78, P = 0.0016; model factor: F_{(1, 8)} = 28.44, P = 0.0007; LD × model: F_{(1, 8)} = 87.11, P < 0.0001; n = 3 per group. C, D Concentration of MCP-1, GM-CSF and M-CSF in rat serum. Two-way ANOVA: MCP-1:LD × model: F_{(1, 14)} = 6.652, P = 0.0218; M-CSF: LD factor: F_{(1, 28)} = 23.57, P < 0.0001; GM-CSF: LD factor: F_{(1, 28)} = 12.88, P = 0.0013; n = 4 to 11 per group