Table 1 Primer sequences for real-time PCR analysis
From: Dark-light cycle disrupts bone metabolism and suppresses joint deterioration in osteoarthritic rats
Gene | Primer sequences | Product size | GenBank accession no |
|---|---|---|---|
BMAL1 | F: 5′-CAGAAGCAAACTACAAGCCAA-3′ | 149 bp | NM_024362 |
R: 5′-GGTCACATCCTACGACAAACA-3′ | |||
NR1D1 | F: 5′-CGGTCTACGGCAAGGCAACAC-3′ | 145 bp | NC_051345 |
R: 5′-TTCTACCACCTCCCGCACAGC-3′ | |||
CRY1 | F: 5′-GTGGTGGCGGAAACTGCTCTC-3′ | 146 bp | NC_051342 |
R: 5′-TGCGTCCTCTTCCTGACTTGGG-3′ | |||
PER3 | F: 5′-CAACCGCACCATCCGCAGAC-3′ | 82 bp | NM_023978 |
R: 5′-CTTACGCCAGACGCCATGCTC-3′ | |||
GAPDH | F: 5′-GATGCCCCCATGTTTGTGAT-3′ | 150 bp | NC_051339 |
R: 5′-GGCATGGACTGTGGTCATGAG-3′ |