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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Myeloid cell-specific deletion of Capns1 prevents macrophage polarization toward the M1 phenotype and reduces interstitial lung disease in the bleomycin model of systemic sclerosis

Fig. 3

Analyses of lung inflammation, fibrosis and immune cell infiltrates in a mouse of the bleomycin model of SSc. A, C Lung sections of sham treated (Control) or BLM treated (bleomycin model of SSc) WT or Capns1-ko mice were stained with H&E (A), anti-CD8 (C) and F4/80 (C). Scale bars, 50 μm,magnification X200. B, D, E An inflammatory score was determined based on histopathological analysis as described in materials and methods (B), or CD8 T cells (D) or F4/80 macrophages (E) were quantitated using image analysis software. In the WT mice, there was the infiltration of inflammatory cells, widened alveolar septa and pulmonary interstitial fibrosis in lung tissues were found in bleomycin model of SSc group, but no infiltration of inflammatory cells in lung tissues of sham group A. Also, there was no infiltration of inflammatory cells in lung tissues of Capns1-ko mice in bleomycin model of SSc. In bleomycin model of SSc model, the inflammatory score in lung tissues was significantly higher B. Lung tissues containing yellow granulation in the endochylema or nucleus were considered as positive C. The number of CD8 positive cells increased in the WT mice of the bleomycin model of SSc group in comparison to WT mice of sham group, and decreased in the Capns1-ko mice of the bleomycin model of SSc group with significant difference D. The number of F4/80 positive cells increased in the WT mice of the bleomycin model of SSc group in comparison to WT mice of sham group, and decreased in the Capns1-ko mice of the bleomycin model of SSc group with significant difference E. The number of α-SMA positive cells was similar in the WT mice of the bleomycin model of SSc group in comparison to WT mice of sham group, also similar in the Capns1-ko mice of the bleomycin model of SSc group with no significant difference F. The test of Homogeneity of variance showed P < 0.1, nonparametric test was applied. Data are mean ± SD. n = 8-10, *: P < 0.05, vs. sham group in WT mice; #: P < 0.05, vs. bleomycin model of SSc group in WT mice. OD optical density.

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