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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Myeloid cell-specific deletion of Capns1 prevents macrophage polarization toward the M1 phenotype and reduces interstitial lung disease in the bleomycin model of systemic sclerosis

Fig. 4

Analyses of fibrosis in lung tissues in a mouse of the bleomycin model of SSc. A Lung sections of sham treated (Control) or BLM treated (bleomycin model of SSc) WT or Capns1-ko mice were stained with H&E and Masson’s trichrome. Scale bars, 50 μm, magnification X200. B Quantitation of mean collagen staining area of lung sections as shown in A. C The hydroxyproline (HYP) content in lung tissues was determined biochemically and expressed as µg HYP per gram of wet tissue. Masson’s trichrome staining showed a dense deposition of collagen in the pulmonary interstitial tissues in WT mice of the bleomycin model of SSc model, and it significantly increased when the sclerosis was developed in WT mice of the bleomycin model of SSc model A. The collagen area rate in lung tissues in sham group was significantly less than that in the WT mice of the bleomycin model of SSc group B. The HYP content in lung tissues increased in the bleomycin model of SSc group in comparison to sham group with significant difference in WT mice C. In Capns1-ko mice of the bleomycin model of SSc model, the collagen area rate and HYP content in lung tissues were increased significantly (B, C). Data are mean ± SD. n = 8. Also, immunohistochemical staining analyses of TGF-β1, collagen I and collagen III in lung tissues in WT and Capns1-ko mice of the bleomycin model of SSc were done. D Lung sections of sham treated (Control) or BLM treated (bleomycin model of SSc) WT or Capns1-ko mice were stained for TGF-β1, Collagen I or Collagen III Scale bars, 50 μm, magnification X200. E, F, G Mean staining areas were quantified for TGF-β1 (E), Collagen I (F) or Collagen III (G). The test of Homogeneity of variance showed P < 0.1, nonparametric test was applied. Data are mean ± SD. n = 10, *: P < 0.05, vs. sham group in WT mice; #: P < 0.05, vs. bleomycin model of SSc group in WT mice. OD optical density

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