Table 2 Sequence of primers for qRT-PCR
Gene symbol | Forward (5′→3′) | Reverse (5′→3′) |
|---|---|---|
Col2a1 | AGCGACTGTCCCTCGGAAAAAC | CCAGGTAGGCGATGCTGTTCTTAC |
Acan | CCTGCTACTTCATCGACCCC | AGATGCTGTTGACTCGAACCT |
Sox9 | TCAGATGCAGTGAGGAGCAC | CCAGCCACAGCAGTGAGTAA |
Mmp-13 | ACAGGCTCCGAGAAATGCAA | CCACATCAGGCACTCCACAT |
Wnt3a | CCTCTCGGATACCTCTTAGTGC | AGCTGCTTCGGTACCAGG |
Wnt5a | CTGTCTTTGGCAGGGTGATG | TAGTCGATGTTGTCTCCGCA |
Wnt5b | TGTTCATCATTGGCGCTCAG | TACGTGAAGGCAGTCTCTCG |
Wnt16 | CCACTACCACTTCCACCCAG | CTGAACCACATGCCGTACTG |
Catenin | TGATTCGAAACCTTGCCCTT | AGCAAGGATGTGGAGAGCTC |
β-actin | GGCTGTATTCCCCTCCATCG | CCAGTTGGTAACAATGCCATGT |
Hprt1 | CTGGTGAAAAGGACCTCTCGAA | CTGAAGTACTCATTATAGTCAAGGGCAT |