Fig. 3

DNA damage in mouse and human OA chondrocytes. A Representative Western blot images of γH2AX in chondrocytes treated with IL‐1β (1 ng/ml, 36 h) or infected with 800 MOI of Ad-HIF‐2α or Ad-ZIP8 (36 h). ERK was detected as a loading control. B and C Representative images of safranin-O staining and γH2AX immunostaining in mouse OA cartilage induced by DMM surgery (n = 5) (B) and human OA cartilage tissue (n = 5 patients) (C). D Semi-quantification of γH2AX positive pixels determined by the Aperio ImageScope Viewer software (n = 5 mice or patients). Values are presented as mean ± s.e.m., and significance was evaluated by paired t-test. Scale bars: 50 µm