Fig. 2
From: Role of high-temperature requirement serine protease A 2 in rheumatoid inflammation
Expression of HtrA2 in RA synovial fluids and the synovium. A HtrA2 levels in the SF of RA patients with considerable synovial hypertrophy (grayscale US, GSUS 2 and 3) compared with those with little or mild synovial hypertrophy (GSUS 0 and 1). B HtrA2 concentrations in RA patients with and without enhanced vascularity (power-Doppler US, PDUS 1–3). (PDUS 0). C HtrA2 concentrations in RA patients with and without active synovitis. Active synovitis was classified as GSUS ≥2 or PDUS ≥1. D Anti-HtrA2 antibody or isotype control antibody immunohistochemical staining of rheumatoid arthritis (RA, n=4) and osteoarthritis (OA, n=4) synovium. HtrA2 positivity was found in the cell lining layer (arrow), as well as in infiltrating leukocytes in the sublining layer (arrowhead). Scale bar, 20 μm. E Summary of the percentage of cells in each layer. Data are the mean ± s.e.m. *p < 0.05, **p < 0.01, ***p < 0.001. P values were determined by one-way ANOVA test (A–C) or two-way ANOVA (E)