Fig. 2

Identifying gene targets of miR-335-5p. A Heatmap from unsupervised clustering of miR-335-5p gene target expression changes following miR-335-5p inhibition in late-stage knee OA fat pad (n = 3). Two genes (COL10A1 and WNT10A) with undetermined Ct values were not included. B Top 5 gene targets with the largest positive logFC following miR-335-5p inhibition. ΔG = Gibb’s free energy value. C miR-335-5p seed-sequence binding region in VCAM1 predicted by TargetScan 8.0. D, E Expression changes in late-stage knee OA fat pad following miR-335-5p inhibitor versus control inhibitor treatment, and miR-335-5p mimic versus control mimic treatment (n = 6). -5p = miR-335-5p; inh. = inhibitor; mim. = mimic; FC = fold-change; bars = 95% CI; *p < 0.05