Fig. 3

Identifying gene targets of miR-335-3p. A Heatmap from unsupervised clustering of miR-335-3p gene target expression changes following miR-335-3p inhibition in late-stage knee OA fat pad (n = 3). Two genes (COL2A1 and SHH) with undetermined Ct values were not included. B Top 5 gene targets with largest positive logFC following miR-335-3p inhibition. ΔG = Gibb’s free energy value. C, D, and F Expression changes in late-stage knee OA fat pad following miR-335-3p inhibitor versus control inhibitor treatment, and miR-335-3p mimic versus control mimic treatment (n = 6). -3p = miR-335-3p; inh. = inhibitor; mim. = mimic; FC = fold-change; bars = 95% CI; *p < 0.05. E miR-335-3p seed-sequence binding region in MMP13 predicted by TargetScan 8.0