Fig. 1

Lupus pregnancy leads to increased LDG activation in blood. A Representative flow cytometry plots from SLE and HC that illustrate the gating strategy for proportions of CD62L-negative LDG analyzed among PBMC after density centrifugation. Proportions of LDG (B) and proportions of LDG that have shed CD62L (C) in the first, second, and third trimesters (T1, T2, and T3) compared to late postpartum in women with SLE. Proportions of LDG (D) and proportions of LDG that have shed CD62L (E) in pregnant women with SLE compared to pregnant healthy women (combined data from trimesters one to three). F Representative flow cytometry plots regarding CD10 expression on LDG from SLE and HC during pregnancy and SLE postpartum, and comparison of CD10-negative immature LDG in SLE and HC during pregnancy and to postpartum in SLE. *p < 0.05, **p < 0.01, and **** p < 0.0001, B, C, and F Kruskal-Wallis followed by Dunn’s multiple comparison test and D and E Mann-Whitney U test. Illustration created in BioRender.com