Fig. 2

CMS-induced apoptosis of AFCs in vitro. a Representative flow cytometry scatter plots detecting AFCs apoptosis under different degrees of CMS. b Representative Tunel staining fluorescence pictures detecting AFCs apoptosis under different degrees of CMS (scale bar = 50 μm). Apoptotic AFCs appeared red and nuclei were counterstained with Hoechst 33,358 (blue). c Representative MMP staining fluorescence pictures detecting AFCs apoptosis under different degrees of CMS (scale bar = 50 μm). MMP appeared red and nuclei were counterstained with Hoechst 33,358 (blue). d Statistic data of apoptotic AFCs under different degrees of CMS determined by flow cytometry (n = 3). e Statistic data of Tunel-positive apoptotic AFCs under different degrees of CMS determined by Tunel staining (n = 3). f Statistic data of mean MMP fluorescent intensity in AFCs under different degrees of CMS determined by MMP staining (n = 3). g Representative immunofluorescent staining pictures detecting the expression of Piezo1 channel in AFCs of the Ctrl group and CMS group (scale bar = 50 μm). Piezo1 appeared green and nuclei were counterstained with Hoechst 33,358 (blue). h Statistic data of mean Piezo1 fluorescent intensity in AFCs of the Ctrl group and CMS group (n = 3). i Representative Fluo-4 AM staining pictures detecting the Ca²⁺ influx in AFCs of the Ctrl group and CMS group (scale bar = 10 μm). Fluo-4 AM appeared green and nuclei were counterstained with Hoechst 33,358 (blue). j Statistic data of mean Fluo-4 AM fluorescent intensity in AFCs of the Ctrl group and CMS group (n = 3). ^**P < 0. 01, ^***P < 0.001, ^****P < 0.0001