Fig. 1

Drp1 induces gouty arthritis. A The histological changes in the joints were assessed by H&E staining. Scale bar = 50 μm. B The immunoreactivity of Drp1 in the joints was detected by IHC. Scale bar = 50 μm. C, F The mRNA level of Drp1 was detected by RT-qPCR. D The protein levels of Drp1 and USP16 in the joints were detected by western blot. E, I The secretion of IL-1β, TNF-α and IL-6 were measured by ELISA assay. F, J The activities of antioxidant enzymes were detected using commercial kits. H The protein level of Drp1 was detected by western blot. K The LDH release was measured using commercial kit. L The protein levels of Drp1 and USP16 in the synovial tissues were detected by western blot. *, P < 0.05, **, P < 0.01, ***, P < 0.001