Fig. 4From: Deubiquitinase USP16 induces gouty arthritis via Drp1-dependent mitochondrial fission and NLRP3 inflammasome activationDeubiquitinase USP16 directly interacts with Drp1. A The protein level of Drp1 was detected by western blot. The mRNA (B) or protein levels (C) of USP16 and Drp1 were detected by RT-qPCR or western blot, respectively. D The direct interaction between USP16 and Drp1 in macrophages was assessed by Co-IP. Whole cell lysates were used as input control. Normal IgG served as a negative control. E The in vitro interaction between GST-tagged USP16 and Drp1 was detected by GST pull-down assay. Whole cell lysates were used as input control. Vector alone acted as a negative control. *, P < 0.05, **, P < 0.01, ***, P < 0.001Back to article page