Fig. 2
Neutrophil granular enzyme activities and NETs formation among oligo JIA patients. A NE activity, B MPO activity, and C percentage of NETs formation of oligo JIA patients (N = 4) compared to pediatric healthy controls (HC) (N = 6). NE activity and NETs were increased compared to healthy controls at diagnosis and decreased after anti-inflammatory treatment, only the difference between HC and oJIA at diagnosis was statistically significant. Representative immunofluorescent staining demonstrating NETs formation of D healthy control; E oligo JIA patient at diagnosis; F oligo JIA patient after anti-inflammatory treatment. Oligo JIA patients at diagnosis exhibit a significant increase in NETs formation in comparison to healthy control. Following anti-inflammatory treatment NETs formation in oligo JIA patients decreased. White arrows represent NETing neutrophils. G NE activity and H MPO activity showed no correlation with cJADAS. I NETs formation exhibited a statistically significant linear correlation with cJADAS10 (**P = 0.007)