Fig. 7
From: Sirt3 improves monosodium urate crystal-induced inflammation by suppressing Acod1 expression
Acod1 knockdown improved mitochondrial morphology, promoted AMPK activation, and inhibited Drp1 protein expression. a Mitochondrial structure was examined by TEM in the BMDMs. Statistical analysis of the mitochondrial aspect ratio is shown at the bottom. Mitochondria were analyzed in at least 10 fields. b Representative LCM images of Mito-Tracker green probe in live BMDMs mitochondrial imaging. Representative cells in each group surrounded in the white frames were enlarged. The box and whisker plot of the quantified mitochondrial aspect ratio was on the right; DAPI stains nuclei. Scale bar, 40 μm. Mitochondria were analyzed in at least 10 fields. c, d Western blot was used to detect AMPKα, phosphorylation of AMPKα, and phosphorylation of Drp1. *P < 0.05. NS represents no statistical difference