Fig. 6

ALLN administration reduced αSMA-positive cells and CD3-positive leukocytes in the skin of daily bleomycin injections. A αSMA-positive cells suggestive myofibroblasts were quantified by immunohistochemistry in the skin of mice after 28 days of daily bleomycin injections. Representative images are shown (left). Scale bar, 50 μm. Quantitative analysis is shown in the bar graph (right). Values represent mean ± SEM; *, p < 0.05. B The infiltration of CD3-positive T cells and F4/80-positive macrophages were investigated using immunohistochemistry in the skin of mice after 7 days of daily bleomycin injections. Representative images are shown (left). Scale bar, 100 μm. Quantitative analysis is shown in the bar graph (right). Values represent mean ± SEM; *, p ≤ 0.05. C The expression of p-Smad cells was evaluated by immunohistochemistry in the skin of mice after 7 days of daily bleomycin injections. Representative immunohistochemistry images are shown in each group; n = 5 for each group. Scale bar, 100 μm. D Protein expression levels of p-Smad3 were quantified using Western blotting. Values were normalized to GAPDH levels and were shown as relative expressions; n = 3 for each group. Values represent mean ± SEM; *, p < 0.05