Fig. 7
From: A cysteine proteinase inhibitor ALLN alleviates bleomycin-induced skin and lung fibrosis
The effect of ALLN treatment on the expression of calpain 1 and 2 associated with EMT in bleomycin-injected mouse skin. A Skin sections of placebo-and ALLN-treated mice at day 7 after bleomycin injection were immunostained for calpain 1 and 2. Scale bar, 100 μm. Representative immunohistochemistry images are shown in each group. B Protein expression of calpain 1 and calpain 2 in skin samples of placebo- and ALLN-treated mice at day 7 after bleomycin injection were quantitatively analyzed by Western blotting. Values were normalized to tubulin levels and were shown as relative expression values representing mean ± SEM; n = 3 for each group; *, p < 0.05