Fig. 8
From: A cysteine proteinase inhibitor ALLN alleviates bleomycin-induced skin and lung fibrosis
The effect of ALLN treatment on lung fibrosis in subcutaneously bleomycin-injected mouse. Subcutaneous bleomycin injection induces lung inflammation and fibrosis. The antifibrotic effects of ALLN were analyzed in the lung tissues of mice receiving daily subcutaneous injections of bleomycin concurrent with peritoneal injections of ALLN or PBS (three times per week) for 4 weeks. Representative images of H&E and Masson’s trichrome (whole left lung and magnified), αSMA, CD3, F4/80, calpain 1, and calpain 2 staining (magnified) are shown. Scale bar, 50 μm; n = 5 for each group