Fig. 5From: ACSL4 inhibition prevents macrophage ferroptosis and alleviates fibrosis in bleomycin-induced systemic sclerosis modelACSL4 inhibition abolishes overshooting Calpain-mediated ferroptosis. A–D Western blots for Calpain system and ACSL4 in Raw264.7 after CAPN1 or CAPN2 over-expression. E Relative mRNA levels of calpain 1 and calpain 2 in Raw264.7 after CAPN1 or CAPN2 over-expression. F–I Western blots for ferroptosis markers in Raw264.7 after CAPN1 or CAPN2 over-expression with rescued by ROSI (1 μM, 24 h) or not. J, K Relative mRNA levels of ferroptosis markers in Raw264.7 after CAPN1 or CAPN2 over-expression with rescued by ROSI (1 μM, 24 h) or not. L, M Relative MDA levels and net GSH concentrations of Raw264.7 after CAPN1 or CAPN2 over-expression with rescued by ROSI (1 μM, 24 h) or not. (Data are presented as Mean ± SD *p < 0.05, **p < 0.01, ***p < 0.001, * stands for lv-control versus lv-over-expression group; #p < 0.05, ##p < 0.01, ###p < 0.001, #stands for control group versus ROSI incubated group)Back to article page