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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Rspo2 exacerbates rheumatoid arthritis by targeting aggressive phenotype of fibroblast-like synoviocytes and disrupting chondrocyte homeostasis via Wnt/β-catenin pathway

Fig. 2

Increased Rspo2 upregulates the expression of LGR5 and β-catenin in fibroblast-like synoviocytes and chondrocytes. A, B Immunofluorescence staining and statistical analysis of LGR5 (A) and β-catenin (B) in human synovium (n = 8 per group). Scale bar: 25 μm. C Immunofluorescence staining and statistical analysis of LGR5 and β-catenin in the synovium and knee cartilage from sham and RA mice administrated with a vehicle, rmRspo2, Rspo2-NAb, or rmDKK1 for 4 and 8 weeks (n = 6 per group). Scale bar: 12.5 μm and 25 μm. D, E Western blot (D) and quantitative PCR analysis (E) of LGR5 and β-catenin in rhRspo2-treated FLS with or without Rspo2-NAb or rhDKK1 (n = 3 per group). F, G Western blot (F) and quantitative PCR analysis (G) of LGR5 and β-catenin in rhRspo2-treated primary chondrocytes with or without Rspo2-NAb or rmDKK1 (n = 3 per group). Student’s t-test or one-way analysis of variance (ANOVA) and Tukey’s multiple comparison test. *P < 0.05, **P < 0.01; ns, no significance. Data are shown as mean ± SEM

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Arthritis Research & Therapy

ISSN: 1478-6362

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