Fig. 5From: TIGIT reverses IFN-α-promoted Th1-like Tregs via in-sequence effects dependent on STAT4IFN-α improved Th1-like Tregs through STAT4 pathway in-sequence condition. CD4+ T cells were purified from PBMCs of HDs. A Relative messenger RNA expression of STAT1 and STAT4 in transfected CD4+ T cells was evaluated by quantitative PCR. B Knocking out STAT4 before exposing to specified conditions. Bar graphs showing the percentages of CXCR3+Foxp3+Th1-like Tregs analyzed using flow cytometry. C–E CD4+ T cells were activated by TCR before incubated under specified conditions and analyzed using flow cytometry. C Percentages of pSTAT1+ cells, and STAT4+ cells in Foxp3+Tregs. D Percentages of CXCR3+ cells and T-bet+ cells. E MFI of CXCR3 and T-bet in Foxp3+Tregs. Data are mean ± SEM of three independent experiments using different donors. *P < 0.05, **P < 0.01, **P < 0.001Back to article page